Supplementary Figure 1: Immunophenotypic profiling by single-cell mass cytometry. | Nature Neuroscience

Supplementary Figure 1: Immunophenotypic profiling by single-cell mass cytometry.

From: Human microglia regional heterogeneity and phenotypes determined by multiplexed single-cell mass cytometry

Supplementary Figure 1

(a) Two-dimensional projections of single-cell data (Panel B) generated by t-SNE of PBMCs (n =4 biologically independent samples), CSF cells (n = 4 biologically independent samples) and brain mononuclear cells (n = 36 biologically independent samples). Each dot represents one cell. The color spectrum represents expression of TMEM119 (red denotes high expression, blue denotes no expression). TMEM119+ cells were gated as huMG (blue) and TMEM119- cells were gated as different circulating immune cells. (b) Representative scatter plots and histograms (two independently repeated experiments with similar results) show expression level of P2Y12 in TMEM119+ cells (upper panel) and vice versa of TMEM119 in P2Y12+ cells (lower panel). (c) The graph shows the quantitative frequencies of P2Y12+ cells in TMEM119+ cell population (P2Y12+TMEM119+, n = 20 biologically independent samples) and vice versa of TMEM119+ cells in P2Y12+cell population (TMEM119+P2Y12+, n = 20 biologically independent samples). Black lines show mean values of the data sets. The values show mean ± SD.

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