Supplementary Figure 5: Differential immunophenotypes of postmortem and fresh human microglia. | Nature Neuroscience

Supplementary Figure 5: Differential immunophenotypes of postmortem and fresh human microglia.

From: Human microglia regional heterogeneity and phenotypes determined by multiplexed single-cell mass cytometry

Supplementary Figure 5

(a) Heat map and cluster dendrogram demonstrates the mean expression of all analyzed markers and relationships between postmortem GTS-huMG (green), postmortem GFM-huMG (orange) and huMG from fresh biopsies (blue). Heat colours have been scaled per marker (red denotes high and blue denotes low expression). (b) Heat map and cluster dendrogram demonstrates the mean expression of analyzed markers (excluded IRF-8 and P2Y12) and relationships between postmortem GTS-, GFM-huMG and huMG from fresh biopsies, respectively. (c) An overlaid t-SNE plot (left image) of all cells from all samples (green = postmortem GTS-huMG (n = 10), orange = postmortem GFM-huMG (n = 9) and blue = huMG from fresh biopsies (n = 3)). Two main clusters, G1 (blue) and G2 (orange), are detected. Overlaid histograms and single dot plots show mean expression levels of selected markers showing differential marker expressions between the two gates (G1 & G2 in a) in huMG from GTS (green), GFM (orange) and fresh biopsies (blue). Black lines show the mean of data sets.

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