Supplementary Figure 3: Analysis of neural activity using lowered velocity thresholds for resting periods, distribution of timing fields across all temporal encoding neurons, examples of spatial cell activity, and correlations between ΔF/F and velocity, all during (invisible door) Door Stop Task. | Nature Neuroscience

Supplementary Figure 3: Analysis of neural activity using lowered velocity thresholds for resting periods, distribution of timing fields across all temporal encoding neurons, examples of spatial cell activity, and correlations between ΔF/F and velocity, all during (invisible door) Door Stop Task.

From: Evidence for a subcircuit in medial entorhinal cortex representing elapsed time during immobility

Supplementary Figure 3

a. Two examples of mean DF/F versus time across all correct trials in a single session for all neurons in a single FOV during the 6 second door stop wait interval for Mouse 1 (left) and Mouse 2 (right). Data analyzed with upper rest velocity threshold = 5.2 cm/sec (left) and for upper rest velocity threshold = 1.95 cm/sec (right). Mean DF/F normalized to peak for each neuron (each row). Mean DF/F for cells that did not have significant timing fields with lowered velocity threshold were colored visible blue. (left: N = 18 cells from 1 imaging field; right: N = 22 cells from 1 imaging field). b. Distribution of Run (at door)-Rest (at door) Index (see Methods). c. Mean DF/F versus time across all correct trials in the Door Stop task from all time encodings neurons (each row represents single neuron mean DF/F) across all FOVs. Mean DF/F normalized to peak for each neuron (each row). d. Bottom, DF/F versus track location during locomotion for each trial of a single session for 2 individual neurons from the same FOV during Door Stop task running phase. Top, Mean DF/F versus track location across all trials. Purple dashed lines and arrows indicate transition period. e. Mean DF/F versus track location during locomotion across all trials in a single session for all neurons (each row represents single neuron mean DF/F) in a single FOV during the Door Stop task running phase. Mean DF/F normalized to peak for each neuron (each row). f. Histogram of Pearson’s Correlations between mouse velocity and DF/F vs time traces during behavior in the Door Stop task for all active cells (left), temporal encoding and spatial encoding cell (right); transition periods and reward zone excluded.

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