a. Examples of normalized DF/F sequence for all cells from an individual trial for error waits (mean error wait = 5.1 ± 0.5 sec; left), short waits (mean short wait = 6.5 ± 0.3 sec; middle) and long waits (mean long wait = 8.0 ± 0.7 sec; right). Cells were ordered according to each cell’s mean center of mass across all short wait trials. Red, pink and green lines are linear fits of error (red, left), short (pink, middle) and long (green, right) wait sequences (N = 7 cells from 1 imaging field). b. Plot of slope (from linear fits of cell activations per second) as a function of animal wait time for all individual trials (each circle represents a single trial, as seen in a). Cells were ordered according to each cell’s mean COM across all correct (6-9.5 sec) trials. N=86 wait trials from 4 imaging fields in 3 mice.