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ALL-OPTICAL NEUROPHYSIOLOGY

Sculpting light to reveal brain function

New techniques enable simultaneous optogenetic stimulation and calcium imaging from ensembles of tens of neurons in vivo. Improved opsins are localized to the cell body, minimizing spurious activation of the optically unresolvable neuropil. Two-photon light pulses are sculpted in space, time, and wavelength to efficiently target the desired cells.

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Correspondence to Adam E. Cohen.

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Fig. 1: Optical control and readout of neural ensembles with 3D-SHOT.