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ALL-OPTICAL NEUROPHYSIOLOGY

Sculpting light to reveal brain function

Nature Neuroscience volume 21, pages 776–778 (2018)Cite this article

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New techniques enable simultaneous optogenetic stimulation and calcium imaging from ensembles of tens of neurons in vivo. Improved opsins are localized to the cell body, minimizing spurious activation of the optically unresolvable neuropil. Two-photon light pulses are sculpted in space, time, and wavelength to efficiently target the desired cells.

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Fig. 1: Optical control and readout of neural ensembles with 3D-SHOT.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA

    Adam E. Cohen & Samouil L. Farhi

  2. Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA

    Adam E. Cohen

  3. Department of Physics, Harvard University, Cambridge, MA, USA

    Adam E. Cohen

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  1. Adam E. Cohen
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  2. Samouil L. Farhi
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Correspondence to Adam E. Cohen.

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The authors declare no competing interests.

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Cohen, A.E., Farhi, S.L. Sculpting light to reveal brain function. Nat Neurosci 21, 776–778 (2018). https://doi.org/10.1038/s41593-018-0158-5

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