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Decoding microscopy images by accurate measurement of point spread functions

uiPSF is a toolbox to measure point spread functions based on inverse modeling that improves single-molecule localization microscopy (SMLM) localization and microscope characterization, and that works for many microscopy technologies.

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Fig. 1: uiPSF supports versatile PSF modeling for different microscopes, covering both spatial and Fourier domains.

References

  1. Lelek, M. et al. Single-molecule localization microscopy. Nat. Rev. Methods Primers 1, 39 (2021). A review article that presents SMLM.

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  3. Fu, S. et al. Deformable mirror based optimal PSF engineering for 3D super-resolution imaging. Optics Lett. 47, 3031–3034 (2022). This paper reports PSFs with large depth of focus.

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This is a summary of: Liu, S. et al. Universal inverse modeling of point spread functions for SMLM localization and microscope characterization. Nat. Methods https://doi.org/10.1038/s41592-024-02282-x (2024).

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Decoding microscopy images by accurate measurement of point spread functions. Nat Methods 21, 946–947 (2024). https://doi.org/10.1038/s41592-024-02283-w

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