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Decoding microscopy images by accurate measurement of point spread functions

uiPSF is a toolbox to measure point spread functions based on inverse modeling that improves single-molecule localization microscopy (SMLM) localization and microscope characterization, and that works for many microscopy technologies.

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Fig. 1: uiPSF supports versatile PSF modeling for different microscopes, covering both spatial and Fourier domains.


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This is a summary of: Liu, S. et al. Universal inverse modeling of point spread functions for SMLM localization and microscope characterization. Nat. Methods (2024).

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Decoding microscopy images by accurate measurement of point spread functions. Nat Methods 21, 946–947 (2024).

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