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Targeted illumination confocal microscopy enables in vivo voltage imaging in thick tissue

A fundamental mechanism for information processing in the brain is electrical activity. However, observing such activity at the single-cell level is challenging. We have developed an optical microscope that combines the advantages of targeted illumination and confocal gating to enable kilohertz-rate voltage imaging across large fields of view in thick tissue.

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Fig. 1: TICO microscope enables high-SNR voltage imaging at large scales and over long durations.


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This is a summary of: Xiao, S. et al. Large-scale deep tissue voltage imaging with targeted-illumination confocal microscopy. Nat. Methods (2024).

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Targeted illumination confocal microscopy enables in vivo voltage imaging in thick tissue. Nat Methods 21, 948–949 (2024).

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