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A genome-scale approach for determining the function of phosphorylation sites

Combining post-translational modification site-centric base editing with phenotypic screens uncovers the function of phosphorylation sites in high throughput, enabling the study of expansive signaling networks at a speed comparable to that of functional genomics.

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Fig. 1: Identification of kinases that modulate NFAT transcriptional activity during T cell activation.

References

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This is a summary of: Kennedy, P. H. et al. Post-translational modification-centric base editor screens to assess phosphorylation site functionality in high throughput. Nat. Methods https://doi.org/10.1038/s41592-024-02256-z (2024).

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A genome-scale approach for determining the function of phosphorylation sites. Nat Methods 21, 940–941 (2024). https://doi.org/10.1038/s41592-024-02264-z

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