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Quantitative profiling of regulatory DNA activity at single-cell resolution

We developed a two-pronged strategy to functionally probe the enormous repertoire of noncoding DNA within genomes. Our approach markedly improved signal-to-noise ratio and successfully intersected single-cell genomics with reporter assays. The result delivers a multiplex and highly quantitative readout of regulatory sequences’ activity in dynamic and multicellular systems.

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Fig. 1: scQers provide a high-sensitivity readout of CRE activity.


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This is a summary of: Lalanne, J.-B. et al. Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters. Nat. Methods (2024).

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Quantitative profiling of regulatory DNA activity at single-cell resolution. Nat Methods 21, 936–937 (2024).

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