Here we developed synthetic transactivation domains (TADs) built from human mechanosensitive transcription factors (MTFs). By linking MTF TAD segments together, we engineered compact and potent multipartite transcriptional activation modules. We then harnessed these modules to create a CRISPR activation system, which we termed the dCas9 recruited enhanced activation module (CRISPR-DREAM).
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This is a summary of: Mahata, B. et al. Compact engineered human mechanosensitive transactivation modules enable potent and versatile synthetic transcriptional control. Nat. Methods https://doi.org/10.1038/s41592-023-02036-1 (2023).
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Combining compact human protein domains with CRISPR systems for robust gene activation. Nat Methods 20, 1635–1636 (2023). https://doi.org/10.1038/s41592-023-02038-z