CryoREAD automatically builds DNA–RNA atomic structure from cryo-EM maps. Backbone accuracy is typically >85% and the method is applicable for maps with RNA-only, DNA-only and DNA–RNA–protein complex structures. CryoREAD uses deep learning to identify structure information and subsequently construct the 3D structure of nucleic acids.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Kim, M.-S. et al. Cracking the DNA code for V (D) J recombination. Mol. Cell 70, 358–370 (2018). This paper reports the DNA–protein complex structure of HFC.
Thoms, M. et al. Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2. Science 369, 1249–1255 (2020). This paper reports the structure of the SARS-CoV-2-Nsp1–40S complex.
Kappel, K. et al. Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures. Nat. Methods 17, 699–707 (2020). This paper reports a computational method for RNA structure modeling from RNA-only cryo-EM maps.
Terwilliger, T. C. et al. A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps. Nat. Methods 15, 905–908 (2018). This paper reports a computational tool for automated macromolecular structure modeling from high-resolution cryo-EM maps.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Wang, X. et al. CryoREAD: de novo structure modeling for nucleic acids in cryo-EM maps using deep learning. Nat. Methods https://doi.org/10.1038/s41592-023-02032-5 (2023).
Rights and permissions
About this article
Cite this article
CryoREAD provides fully automated DNA–RNA structure modeling for cryo-EM maps. Nat Methods 20, 1637–1638 (2023). https://doi.org/10.1038/s41592-023-02033-4
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41592-023-02033-4
