CryoREAD automatically builds DNA–RNA atomic structure from cryo-EM maps. Backbone accuracy is typically >85% and the method is applicable for maps with RNA-only, DNA-only and DNA–RNA–protein complex structures. CryoREAD uses deep learning to identify structure information and subsequently construct the 3D structure of nucleic acids.
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Kim, M.-S. et al. Cracking the DNA code for V (D) J recombination. Mol. Cell 70, 358–370 (2018). This paper reports the DNA–protein complex structure of HFC.
Thoms, M. et al. Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2. Science 369, 1249–1255 (2020). This paper reports the structure of the SARS-CoV-2-Nsp1–40S complex.
Kappel, K. et al. Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures. Nat. Methods 17, 699–707 (2020). This paper reports a computational method for RNA structure modeling from RNA-only cryo-EM maps.
Terwilliger, T. C. et al. A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps. Nat. Methods 15, 905–908 (2018). This paper reports a computational tool for automated macromolecular structure modeling from high-resolution cryo-EM maps.
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This is a summary of: Wang, X. et al. CryoREAD: de novo structure modeling for nucleic acids in cryo-EM maps using deep learning. Nat. Methods https://doi.org/10.1038/s41592-023-02032-5 (2023).
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CryoREAD provides fully automated DNA–RNA structure modeling for cryo-EM maps. Nat Methods 20, 1637–1638 (2023). https://doi.org/10.1038/s41592-023-02033-4