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Mapping deformations and increasing quantitative accuracy in expansion microscopy

We introduce GelMap, a flexible workflow for reporting deformations and anisotropy in expansion microscopy. By intrinsically calibrating the expansion hydrogel using a fluorescent grid that scales with expansion and deforms with anisotropy, GelMap enables the reliable quantification of expansion factors and correction of deformations.

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Fig. 1: Reporting and correcting deformations using GelMap.


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This is a summary of: Damstra, H. G. J. et al. GelMap: intrinsic calibration and deformation mapping for expansion microscopy. Nat. Methods (2023).

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Mapping deformations and increasing quantitative accuracy in expansion microscopy. Nat Methods 20, 1460–1461 (2023).

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