To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.
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Turk, M. & Baumeister, W. The promise and the challenges of cryo-electron tomography. FEBS Lett. 594, 3243–3261 (2020). A review summarizing the emergence of cryo-ET in structural biology, recent technology developments and future directions.
Berger, C. et al. Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology. Nat. Methods 20, 499–511 (2023). A review summarizing the impact of cryo-ET on structural biology, including the application of FIB milling to cryo-ET workflows and other technology developments.
Schwartz, C. L. et al. Cryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching. J. Microsc. 227, 98–109 (2007). This paper discusses the spatial scale differences between light microscopy and cryo-EM in the cryo-CLEM (correlative light and electron microscopy) workflow.
Peck, A. et al. Montage electron tomography of vitrified specimens. J. Struct. Biol. 214, 107860 (2022). This paper experimented with montage cryo-ET to obtain a single montage tilt series using very restricted beam size to support an efficient hexagonal tiling pattern, which will minimize radiation damage.
Eisenstein, F. et al. Parallel cryo electron tomography on in situ lamellae. Nat. Methods 20, 131–138 (2023). This paper presents a method for collecting tilt series in parallel via an image shift and geometry-based sample model and iteratively refined tracking and autofocus.
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This is a summary of: Yang, J. E. et al. Correlative montage parallel array cryo-tomography for in situ structural cell biology. Nat. Methods https://doi.org/10.1038/s41592-023-01999-5 (2023).
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Capturing detailed cellular landscapes by montage cryo-electron tomography. Nat Methods 20, 1458–1459 (2023). https://doi.org/10.1038/s41592-023-02000-z