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Capturing detailed cellular landscapes by montage cryo-electron tomography

To capture expansive, seamless fields of view from frozen hydrated specimens by cryo-electron tomography, we developed methods for the collection and processing of montage data. This approach enables rapid acquisition of contiguous regions of specimens using a montaged tilt series collection scheme.

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Fig. 1: Montage parallel array cryo-tomography (MPACT).


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This is a summary of: Yang, J. E. et al. Correlative montage parallel array cryo-tomography for in situ structural cell biology. Nat. Methods (2023).

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Capturing detailed cellular landscapes by montage cryo-electron tomography. Nat Methods 20, 1458–1459 (2023).

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