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LIONESS enables 4D nanoscale reconstruction of living brain tissue

We developed LIONESS, a technology that leverages improvements to optical super-resolution microscopy and prior information on sample structure via machine learning to overcome the limitations (in 3D-resolution, signal-to-noise ratio and light exposure) of optical microscopy of living biological specimens. LIONESS enables dense reconstruction of living brain tissue and morphodynamics visualization at the nanoscale.

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Fig. 1: Dynamic nanoscale reconstruction of living brain tissue.


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This is a summary of: Velicky, P. et al. Dense 4D nanoscale reconstruction of living brain tissue. Nat. Methods (2023).

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LIONESS enables 4D nanoscale reconstruction of living brain tissue. Nat Methods 20, 1141–1142 (2023).

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