Multiplexed spatial immunophenotyping has advanced our understanding of tissues in the context of homeostasis and disease. Two studies now provide additional tools to overcome challenges with multiplexed imaging: one procedure amplifies the detection of low-abundance antigens by integrating SABER and IMC technologies, and the other is an X-ray-based method that enables the non-destructive multiplexed detection of antigens in tissues at scalable resolution and speed.
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N.F.C.C.d.M. is funded by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (grant agreement no. 852832).
The authors declare no competing interests.
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Ijsselsteijn, M.E., de Miranda, N.F.C.C. Advancing multiplexed imaging for enhanced tissue complexity analysis. Nat Methods 20, 1280–1281 (2023). https://doi.org/10.1038/s41592-023-01935-7