Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Briefing
  • Published:

Photoselective sequencing harnesses microscopy to guide genomic analyses

Nature Methods volume 20, pages 647–648 (2023)Cite this article

Subjects

Photoselective sequencing is a new method for genomic and epigenomic profiling within specific regions of a biological specimen that are chosen using light microscopy. This combination of spatial and sequencing information preserves the connections between genomic and environmental properties and deepens our understanding of structure–function relationships in cells and tissues.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Unblocking libraries in cell nuclei.

References

  1. Tian, L., Chen, F. & Macosko, E. Z. The expanding vistas of spatial transcriptomics. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01448-2 (2022). This review article overviews spatial omics technologies.

    Article  PubMed  Google Scholar 

  2. Deng, Y. et al. Spatial profiling of chromatin accessibility in mouse and human tissues. Nature 609, 375–383 (2022). This paper reports a spatial barcoding strategy for ATAC-seq.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Payne, A. C. et al. In situ genome sequencing resolves DNA sequence and structure in intact biological samples. Science 372, 6532 (2022). This paper reports a strategy for visualizing genome structure using in situ sequencing.

    Google Scholar 

  4. Li, N. et al. Tn5 transposase applied in genomics research. Int. J. Mol. Sci. 21, 8329 (2020). This paper reviews genomic measurements using Tn5 transposase.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Lareau, C. A. et al. Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility. Nat. Biotechnol. 37, 916–924 (2019). This paper reports a single-cell ATAC-seq atlas of the mouse brain.

    Article  CAS  PubMed  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Mangiameli, S. M. et al. Photoselective sequencing: microscopically guided genomic measurements with subcellular resolution. Nat. Methods https://doi.org/10.1038/s41592-023-01845-8 (2023).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Photoselective sequencing harnesses microscopy to guide genomic analyses. Nat Methods 20, 647–648 (2023). https://doi.org/10.1038/s41592-023-01849-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-023-01849-4

Search

Advanced search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research