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Cell biology

Line-scanning speeds up Brillouin microscopy

Two new Brillouin microscopes leverage line-scanning to overcome previous limitations of the technique, enabling fast imaging, with low phototoxicity, of mechanical properties in living embryos of model organisms and tumor spheroids.

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Fig. 1: Principle of line-scanning Brillouin microscopy.


  1. Ayad, N. M. E., Kaushik, S. & Weaver, V. M. Phil. Trans. R. Soc. Lond. B 374, 20180215 (2019).

    Article  CAS  Google Scholar 

  2. Phillip, J. M., Aifuwa, I., Walston, J. & Wirtz, D. Annu. Rev. Biomed. Eng. 17, 113–141 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Bevilacqua, C. et al. Nat. Methods (2023).

  4. Zhang, J., Nikolic, M., Tanner, K. & Scarcelli, G. Nat. Methods (2023).

  5. Brillouin, L. Ann. Phys. 9, 88–122 (1922).

    Article  Google Scholar 

  6. Mandelstam, L. Zh. Russ. Fiz. Khim. Ova. 58, 381–386 (1926).

    Google Scholar 

  7. Gross, E. Nature 126, 201–202 (1930).

    Article  Google Scholar 

  8. Dil, J. G. Rep. Prog. Phys. 45, 285 (1982).

    Article  Google Scholar 

  9. Prevedel, R., Diz-Muñoz, A., Ruocco, G. & Antonacci, G. Nat. Methods 16, 969–977 (2019).

    Article  CAS  PubMed  Google Scholar 

  10. Antonacci, G. et al. Biophys. Rev. 12, 615–624 (2020).

    Article  PubMed  PubMed Central  Google Scholar 

  11. Elosegui-Artola, A. et al. Nat. Mater. 22, 117–127 (2023).

    Article  CAS  PubMed  Google Scholar 

  12. Zhang, J., Fiore, A., Yun, S.-H., Kim, H. & Scarcelli, G. Sci. Rep. 6, 35398 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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N.K. was supported by a Leverhulme Trust project grant (RPG-2020-068) awarded to Y.M. G.P. was supported by an EMBO Long-Term Fellowship (ALTF 786-2020). Y.M. was funded by an MRC award MR/W027437/1, a Lister Institute Research Prize and the EMBO Young Investigator Programme.

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Correspondence to Yanlan Mao.

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Khalilgharibi, N., Paci, G. & Mao, Y. Line-scanning speeds up Brillouin microscopy. Nat Methods 20, 643–644 (2023).

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