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Single-cell measurement of dynamic protein secretion and transcriptome

Unlike cell surface proteins, secreted proteins are difficult to quantify and trace back to individual cells. We show that the capture of secreted proteins onto their source cell surfaces using an affinity matrix enables simultaneous measurement of protein secretion, cell surface proteins and transcriptomics in thousands of cells at single-cell resolution.

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Fig. 1: TRAPS-seq.


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This is a summary of: Wu, T., Womersley, H. J., Wang, J. R., Scolnick, J. & Cheow, L. F. Time-resolved assessment of single-cell protein secretion by sequencing. Nat. Methods (2023).

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Single-cell measurement of dynamic protein secretion and transcriptome. Nat Methods 20, 651–652 (2023).

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