Unlike cell surface proteins, secreted proteins are difficult to quantify and trace back to individual cells. We show that the capture of secreted proteins onto their source cell surfaces using an affinity matrix enables simultaneous measurement of protein secretion, cell surface proteins and transcriptomics in thousands of cells at single-cell resolution.
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References
Uhlen, M. et al. The human secretome. Sci. Signal. 12, eaaz0274 (2019). This review article summarizes the variety and functions of the human secretome.
Manz, R. et al. Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. Proc. Natl Acad. Sci. USA 92, 1921–1925 (1995). This paper presents the foundation assay for TRAPS-seq: the secretion capture assay.
Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat. Methods 14, 865–868 (2017). This paper shows surface protein measurements combined with single-cell sequencing.
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This is a summary of: Wu, T., Womersley, H. J., Wang, J. R., Scolnick, J. & Cheow, L. F. Time-resolved assessment of single-cell protein secretion by sequencing. Nat. Methods https://doi.org/10.1038/s41592-023-01841-y (2023).
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Single-cell measurement of dynamic protein secretion and transcriptome. Nat Methods 20, 651–652 (2023). https://doi.org/10.1038/s41592-023-01842-x
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DOI: https://doi.org/10.1038/s41592-023-01842-x
