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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation

We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.

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Fig. 1: Lightsheet microscopy of H2B-mScarlet3-labeled cell nuclei in a developing zebrafish larva.


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This is a summary of: Gadella, T. W. J. Jr. et al. mScarlet3: a brilliant and fast-maturing red fluorescent protein. Nat. Methods (2023).

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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation. Nat Methods 20, 497–498 (2023).

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