We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.
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References
Chudakov, D. M., Matz, M. V., Lukyanov, S. & Lukyanov, K. A. Fluorescent proteins and their applications in imaging living cells and tissues. Physiol. Rev. 90, 1103–1163 (2010). This review gives an excellent introduction to fluorescent proteins and their molecular buildup, evolution and applications.
Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Natl Acad. Sci. USA 99, 7877–7882 (2002). This paper describes the extensive mutagenesis needed to create a monomeric red fluorescent protein from the tetrameric, naturally occurring DsRed.
Bindels, D. S. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat. Methods 14, 53–56 (2017). This paper describes the generation of the mScarlet series of enhanced red fluorescent proteins, starting from the mRed7 synthetic template.
Goedhart, J. et al. Bright cyan fluorescent protein variants identified by fluorescence lifetime screening. Nat. Methods 7, 137–139 (2010). This paper describes the lifetime image screening method used to develop the mTurquoise bright cyan fluorescent protein from SCFP3A.
Bindels, D. S., Postma, M., Haarbosch, L., van Weeren, L. & Gadella, T. W. J. Jr. Multiparameter screening method for developing optimized red-fluorescent proteins. Nat. Protoc. 15, 450–478 (2020). This protocol paper describes a method for screening for enhanced red fluorescent proteins.
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This is a summary of: Gadella, T. W. J. Jr. et al. mScarlet3: a brilliant and fast-maturing red fluorescent protein. Nat. Methods https://doi.org/10.1038/s41592-023-01809-y (2023).
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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation. Nat Methods 20, 497–498 (2023). https://doi.org/10.1038/s41592-023-01810-5
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DOI: https://doi.org/10.1038/s41592-023-01810-5
