Stimulated Raman scattering (SRS) microscopy has the capability to simultaneously visualize the spatial distribution of different biomolecules, but it remains challenging to reach super-resolution. To achieve this goal, a deconvolution algorithm, A-PoD, was developed and combined with SRS microscopy, enabling examination of nanoscopic biomolecular distribution and subcellular metabolic activity in cells and tissues.
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This is a summary of: Jang, H. et al. Super-resolution SRS microscopy with A-PoD. Nat. Methods https://doi.org/10.1038/s41592-023-01779-1 (2023).
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A deconvolution algorithm to achieve super-resolution stimulated Raman scattering imaging. Nat Methods 20, 361–362 (2023). https://doi.org/10.1038/s41592-023-01780-8