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A deconvolution algorithm to achieve super-resolution stimulated Raman scattering imaging

Stimulated Raman scattering (SRS) microscopy has the capability to simultaneously visualize the spatial distribution of different biomolecules, but it remains challenging to reach super-resolution. To achieve this goal, a deconvolution algorithm, A-PoD, was developed and combined with SRS microscopy, enabling examination of nanoscopic biomolecular distribution and subcellular metabolic activity in cells and tissues.

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Fig. 1: Deconvolution of SRS images.


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This is a summary of: Jang, H. et al. Super-resolution SRS microscopy with A-PoD. Nat. Methods (2023).

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A deconvolution algorithm to achieve super-resolution stimulated Raman scattering imaging. Nat Methods 20, 361–362 (2023).

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