Stimulated Raman scattering (SRS) microscopy has the capability to simultaneously visualize the spatial distribution of different biomolecules, but it remains challenging to reach super-resolution. To achieve this goal, a deconvolution algorithm, A-PoD, was developed and combined with SRS microscopy, enabling examination of nanoscopic biomolecular distribution and subcellular metabolic activity in cells and tissues.
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Freudiger, C. W. et al. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science 322, 1857–1861 (2008). This article reported SRS microscopy.
Shi, L. et al. Optical imaging of metabolic dynamics in animals. Nat. Commun. 9, 2995 (2018). The article introduces DO-SRS microscopy.
Shi, L. et al. Super-resolution vibrational imaging using expansion stimulated Raman scattering microscopy. Adv. Sci. 9, 220315 (2022). This article introduces super-resolution expansion-SRS microscopy.
Sage, D. et al. DeconvolutionLab2: an open-source software for deconvolution microscopy. Methods 115, 28–41 (2017). The paper provides an overview of the most general deconvolution algorithms and their theoretical backgrounds.
Martinez, S. et al. Superresolution method for a single wide-field image deconvolution by superposition of point sources. J. Microsc. 275, 51–65 (2019). This paper presents the SUPPOSe algorithm, a super-resolution deconvolution method.
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This is a summary of: Jang, H. et al. Super-resolution SRS microscopy with A-PoD. Nat. Methods https://doi.org/10.1038/s41592-023-01779-1 (2023).
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A deconvolution algorithm to achieve super-resolution stimulated Raman scattering imaging. Nat Methods 20, 361–362 (2023). https://doi.org/10.1038/s41592-023-01780-8