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Making single-cell proteomics biologically relevant

Nature Methods volume 20, pages 320–323 (2023)Cite this article

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Recent technological advances in mass spectrometry promise to add single-cell proteomics to the biologist’s toolbox. Here we discuss the current status and what is needed for this exciting technology to lead to biological insight — alone or as a complement to other omics technologies.

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Fig. 1: The dawn of single-cell proteomics.
Fig. 2: Approaching biology with sc-proteomics.

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Acknowledgements

We thank the members of our single-cell and DVP teams in Munich and Copenhagen for discussions. The authors are supported by grants from the Max Planck Society for the Advancement of Science, Germany, and the European Union’s Horizon 2020 research and innovation program ISLET (No. 874839). F.A.R. is an EMBO postdoctoral fellowship holder (ALTF 399-2021). We thank Juliet Percival (Percival Press, Durham, UK) for the scientific illustrations.

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Author notes

  1. These authors contributed equally: Florian A. Rosenberger, Marvin Thielert.

Authors and Affiliations

  1. Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany

    Florian A. Rosenberger, Marvin Thielert & Matthias Mann

  2. Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Matthias Mann

Authors
  1. Florian A. Rosenberger
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  2. Marvin Thielert
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  3. Matthias Mann
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Contributions

M.M., M.T. and F.A.R. conceived the original idea. M.M., F.A.R. and M.T. wrote and edited the original draft. F.A.R., M.T. and M.M. conceptualized and guided the illustration process. All authors wrote, edited and gave final approval to the manuscript.

Corresponding author

Correspondence to Matthias Mann.

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Competing interests

M.M. is an indirect shareholder in Evosep Biosystems. The remaining authors declare no competing interests.

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Rosenberger, F.A., Thielert, M. & Mann, M. Making single-cell proteomics biologically relevant. Nat Methods 20, 320–323 (2023). https://doi.org/10.1038/s41592-023-01771-9

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