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Towards higher-resolution and in vivo understanding of lncRNA biogenesis and function

Nature Methods volume 19, pages 1152–1155 (2022)Cite this article

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Recent studies have revealed multifaceted roles of long noncoding RNAs (lncRNAs) in gene regulation, accompanying an increased understanding of lncRNA processing, localization, interacting macromolecules and structural modules. Here, progress and recently developed technological advances for understanding lncRNA biogenesis, modes of action and cellular phenotypes are highlighted, and challenges and opportunities towards higher-resolution and in vivo studies in this field are discussed.

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Fig. 1: The complex life of lncRNA and lncRNP.

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Acknowledgements

The author is grateful to L. Yang at Fudan University and G. Carmichael at UConn Health for reading the paper. This work is supported by the CAS Project for Young Scientists in Basic Research (YSBR-009), the Shanghai Municipal Commission for Science and Technology (20JC1410300), the National Natural Science Foundation of China (31821004, 31830108, 31725009 and 91940303) and the HHMI International Program (55008728). L.-L.C. acknowledges support from the Xplorer Prize.

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  1. State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China

    Ling-Ling Chen

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Correspondence to Ling-Ling Chen.

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Chen, LL. Towards higher-resolution and in vivo understanding of lncRNA biogenesis and function. Nat Methods 19, 1152–1155 (2022). https://doi.org/10.1038/s41592-022-01626-9

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