Scanning transmission electron microscopy (STEM) techniques reveal atomic-resolution details of organic and inorganic materials. The application of STEM to biological vitrified specimens under low-dose cryogenic imaging conditions demonstrates that STEM also achieves near-atomic-resolution 3D structures of biological macromolecules.
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References
Cheng, Y., Grigorieff, N., Penczek, P. A. & Walz, T. A primer to single-particle cryo-electron microscopy. Cell 161, 438–449 (2015). A review article that presents the advances and basic workflow of the single-particle cryo-EM technique.
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This is a summary of: Lazić, I. et al. Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution. Nat. Methods https://doi.org/10.1038/s41592-022-01586-0 (2022)
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Obtaining cryo-EM structures by scanning transmission electron microscopy. Nat Methods 19, 1179–1180 (2022). https://doi.org/10.1038/s41592-022-01587-z
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DOI: https://doi.org/10.1038/s41592-022-01587-z
