Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

MICROSCOPY

Fluorophores’ talk turns them dark

Dipole–dipole crosstalk between fluorophores separated by a distance of less than 10 nm induces changes in their photophysics, which adds a challenge to localization microscopy in the sub-10-nm regime.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Fig. 1: Microscopy with single molecules: basics and emerging challenges at small length scales.

References

  1. Abbe, E. Arch. Mikrosc. Anat. 9, 413–468 (1873).

    Article  Google Scholar 

  2. Legant, W. R. et al. Nat. Methods 13, 359–365 (2016).

    Article  Google Scholar 

  3. Sieben, C., Banterle, N., Douglass, K. M., Gönczy, P. & Manley, S. Nat. Methods 15, 777–780 (2018).

    CAS  Article  Google Scholar 

  4. Otterstrom, J. et al. Nucleic Acids Res. 47, 8470–8484 (2019).

    CAS  Article  Google Scholar 

  5. Möckl, L. et al. Dev. Cell 50, 57–72.e6 (2019).

    Article  Google Scholar 

  6. Lelek, M. et al. Nat. Rev. Methods Primers 1, 39 (2021).

    CAS  Article  Google Scholar 

  7. von Diezmann, L., Shechtman, Y. & Moerner, W. E. Chem. Rev. 117, 7244–7275 (2017).

    Article  Google Scholar 

  8. Weber, M. et al. Nat. Photonics 15, 361–366 (2021).

    CAS  Article  Google Scholar 

  9. Zhang, Y. et al. Nat. Methods 17, 225–231 (2020).

    CAS  Article  Google Scholar 

  10. Helmerich, D. A. et al. Nat. Methods https://doi.org/10.1038/s41592-022-01548-6 (2022).

    Article  Google Scholar 

  11. Schnitzbauer, J., Strauss, M. T., Schlichthaerle, T., Schueder, F. & Jungmann, R. Nat. Protoc. 12, 1198–1228 (2017).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Leonhard Möckl.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Almahayni, K., Spiekermann, M. & Möckl, L. Fluorophores’ talk turns them dark. Nat Methods 19, 932–933 (2022). https://doi.org/10.1038/s41592-022-01565-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-022-01565-5

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing