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Fluorophores’ talk turns them dark

Dipole–dipole crosstalk between fluorophores separated by a distance of less than 10 nm induces changes in their photophysics, which adds a challenge to localization microscopy in the sub-10-nm regime.

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Fig. 1: Microscopy with single molecules: basics and emerging challenges at small length scales.


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Correspondence to Leonhard Möckl.

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Almahayni, K., Spiekermann, M. & Möckl, L. Fluorophores’ talk turns them dark. Nat Methods 19, 932–933 (2022).

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