Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.


Uncovering ubiquitous protein lactylation

A diagnostic fragment ion in tandem mass spectrometry enables confident protein lactylation assignment and the discovery of broad lysine modification beyond histones.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Fig. 1: Protein lactylation.


  1. Ferguson, B. S. et al. Eur. J. Appl. Physiol. 118, 691–728 (2018).

    Article  CAS  Google Scholar 

  2. Certo, M., Tsai, C. H., Pucino, V., Ho, P. C. & Mauro, C. Nat. Rev. Immunol. 21, 151–161 (2021).

    Article  CAS  Google Scholar 

  3. Zhang, D. et al. Nature 574, 575–580 (2019).

    Article  CAS  Google Scholar 

  4. Wan, N. et al. Nat. Methods (2022).

    Article  PubMed  Google Scholar 

  5. Muroski, J. M., Fu, J. Y., Nguyen, H. H., Ogorzalek Loo, R. R. & Loo, J. A. Proteomics 21, e2000111 (2021).

    Article  Google Scholar 

Download references


This project was funded by NIH grant 1RF1AG064250.

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Xiaofeng Wu or W. Andy Tao.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wu, X., Tao, W.A. Uncovering ubiquitous protein lactylation. Nat Methods 19, 793–794 (2022).

Download citation

  • Published:

  • Issue Date:

  • DOI:


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing