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Spectral phasor analysis enables multiplexed microscopy with bioluminescent probes

Bioluminescent phasor is a new technology for multiplexed, excitation-free imaging at the microscale using luciferase–luciferin pairs. This platform can readily unmix the broad, overlapping emission spectra of bioluminescent reporters, making possible the dynamic tracking of cellular and molecular features over prolonged time periods.

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Fig. 1: Bioluminescent phasor.


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This is a summary of: Yao, Z. et al. Multiplexed bioluminescence microscopy via phasor analysis. Nat. Methods (2022)

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Spectral phasor analysis enables multiplexed microscopy with bioluminescent probes. Nat Methods 19, 801–802 (2022).

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