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All-in-one approach to culture, image and quantify large numbers of organoids at all scales

We developed a streamlined approach coupling microfabricated cell culture substrates, 3D single-objective light sheet imaging and artificial intelligence quantifications to characterize the variability of morphologies of small organoids. Arrayed organoids can be imaged in 3D at around 100 organoids per hour.

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Fig. 1: JeWell chip arrays of organoids for single-objective light sheet imaging.

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This is a summary of: Beghin, A. et al. Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification. Nat. Methods https://doi.org/10.1038/s41592-022-01508-0 (2022).

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All-in-one approach to culture, image and quantify large numbers of organoids at all scales. Nat Methods 19, 799–800 (2022). https://doi.org/10.1038/s41592-022-01509-z

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