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SEQUENCING

Single-cell, single-organoid phenotypic landscapes

A droplet microfluidic system enables deterministic capture and subsequent sequencing and analysis of single-cell transcriptomes from organoids and other small, individual tissue samples.

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Fig. 1: Deterministic, mRNA-capture bead and cell co-encapsulation (DisCo) to analyze single intestinal organoid phenotypes.

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Correspondence to J. Gray Camp.

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J.G.C. and I.L. are employed by F. Hoffmann La Roche.

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Camp, J.G., Lukonin, I. Single-cell, single-organoid phenotypic landscapes. Nat Methods 19, 280–281 (2022). https://doi.org/10.1038/s41592-022-01416-3

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