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Single-cell, single-organoid phenotypic landscapes

Nature Methods volume 19, pages 280–281 (2022)Cite this article

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A droplet microfluidic system enables deterministic capture and subsequent sequencing and analysis of single-cell transcriptomes from organoids and other small, individual tissue samples.

Biological organization emerges in multicellular systems through the push-and-pull interactions of each individual cell. There are enumerable scenarios in biological and biomedical research where one would want to understand the molecules that are abundant in every cell within a biological system. It remains a technical challenge to analyze each cell within an organism, tissue or other complex multicellular system. This challenge is relevant across all biosystems, from Escherichia coli to Loxodonta africana to Homo sapiens1.

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Fig. 1: Deterministic, mRNA-capture bead and cell co-encapsulation (DisCo) to analyze single intestinal organoid phenotypes.

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Authors and Affiliations

  1. Roche Institute for Translational Bioengineering (ITB), Roche Pharma Research and Early Development, Roche Innovation Center Basel, Basel, Switzerland

    J. Gray Camp & Ilya Lukonin

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  1. J. Gray Camp
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  2. Ilya Lukonin
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Correspondence to J. Gray Camp.

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Competing interests

J.G.C. and I.L. are employed by F. Hoffmann La Roche.

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Camp, J.G., Lukonin, I. Single-cell, single-organoid phenotypic landscapes. Nat Methods 19, 280–281 (2022). https://doi.org/10.1038/s41592-022-01416-3

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