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Uncovering an overlooked consequence of phosphorylation: change in cysteine reactivity

Global profiling of changes in the reactivity of cysteine residues in response to phosphorylation during mitosis identifies cysteine residues as potential regulatory and drug binding sites on proteins.

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Fig. 1: Chemical profiling of changes in cysteine reactivity upon protein phosphorylation.

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References

  1. Vinogradova, E. V. et al. Cell 182, 1009–1026.e29 (2020).

    Article  CAS  Google Scholar 

  2. Backus, K. M. et al. Nature 534, 570–574 (2016).

    Article  CAS  Google Scholar 

  3. Maurais, A. J. & Weerapana, E. Curr. Opin. Chem. Biol. 50, 29–36 (2019).

    Article  CAS  Google Scholar 

  4. Kemper, E. K. et al. Nat. Methods https://doi.org/10.1038/s41592-022-01398-2 (2022).

  5. Zanon, P. R. A., Lewald, L. & Hacker, S. M. Angew. Chem. Int. Edn Engl. 59, 2829–2836 (2020).

    Article  CAS  Google Scholar 

  6. Sharma, K. et al. Cell Rep. 8, 1583–1594 (2014).

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National Institutes of Health grants R01 EB-028628, R01 EB026332, R01 EB026285 and R01 DK130293 to M.B. and a postdoctoral research fellowship by the German Research Foundation (DFG) to M.L.

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Correspondence to Markus Lakemeyer or Matthew Bogyo.

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Lakemeyer, M., Bogyo, M. Uncovering an overlooked consequence of phosphorylation: change in cysteine reactivity. Nat Methods 19, 281–283 (2022). https://doi.org/10.1038/s41592-022-01414-5

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  • DOI: https://doi.org/10.1038/s41592-022-01414-5

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