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NEUROSCIENCE

Flexibility in mesoscopic imaging

Nature Methods volume 19, page 32 (2022)Cite this article

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Two microscopy approaches bring flexibility to mesoscopic imaging in the brain, allowing independent imaging in multiple regions simultaneously.

Smith’s Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p) combines a roughly 25 mm2 field of view with adaptive optics to achieve subcellular resolution throughout its field of view (Yu et al., 2021). Its two light paths can be operated independently, which allows imaging of separate areas within the larger field of view with different scanning parameters. “Having independence in the two beams is critical for more optimal sampling of extended neural circuitry. It’s also critical for advanced optogenetics experiments,” says Smith. Smith credits his trainees, the “wildly talented” Jeffrey Stirman and Che-Hang Yu, “who can design and build anything,” for starting and finishing the project, respectively.

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Research papers

  • Clough, M. et al. Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds. Nat. Commun. 12, 6638 (2021).

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  • Yu, C.-H. et al. Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry. Nat. Commun. 12, 6639 (2021).

    Article  CAS  Google Scholar 

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  1. Nature Methods http://www.nature.com/nmeth

    Nina Vogt

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  1. Nina Vogt
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Correspondence to Nina Vogt.

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Vogt, N. Flexibility in mesoscopic imaging. Nat Methods 19, 32 (2022). https://doi.org/10.1038/s41592-021-01382-2

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  • DOI: https://doi.org/10.1038/s41592-021-01382-2

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