Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

PROTEOMICS

Towards reproducibility in large-scale analysis of protein–protein interactions

Nature Methods volume 18, pages 720–721 (2021)Cite this article

Subjects

Co-fractionation mass spectrometry (CF-MS) has the potential to measure thousands of protein complexes in a single experiment, but the field is still in its infancy. A meta-analysis of CF-MS data yields a core CF-MS interactome and a tool allowing researchers to align new results to published data.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Three approaches for mapping protein–protein interactions.

References

  1. Skinnider, M.A. & Foster, L.J. Nat. Methods https://doi.org/10.1038/s41592-021-01194-4 (2021).

  2. Hein, M. Y. et al. Cell 163, 712–723 (2015).

    Article  CAS  Google Scholar 

  3. Huttlin, E. L. et al. Nature 545, 505–509 (2017).

    Article  CAS  Google Scholar 

  4. Oughtred, R. et al. Protein Sci. 30, 187–200 (2021).

    Article  CAS  Google Scholar 

  5. Kristensen, A. R., Gsponer, J. & Foster, L. J. Nat. Methods 9, 907–909 (2012).

    Article  CAS  Google Scholar 

  6. Wan, C. et al. Nature 525, 339–344 (2015).

    Article  CAS  Google Scholar 

  7. Bradbury, A. & Plückthun, A. Nature 518, 27 (2015).

  8. Sikorski, K. et al. Nat. Methods 15, 909–912 (2018).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank Johanna Olweus for critical reading of the manuscript. F.L.-J. is partner in the National Network for Advance Proteomics Infrastructure (NAPI), which has received funding from the Norwegian Research Council.

Author information

Authors and Affiliations

  1. Department of Immunology, Oslo University Hospital, Oslo, Norway

    Fridtjof Lund-Johansen, Trung Tran & Adi Mehta

Authors
  1. Fridtjof Lund-Johansen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Trung Tran
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Adi Mehta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fridtjof Lund-Johansen.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lund-Johansen, F., Tran, T. & Mehta, A. Towards reproducibility in large-scale analysis of protein–protein interactions. Nat Methods 18, 720–721 (2021). https://doi.org/10.1038/s41592-021-01202-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-021-01202-7

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing