Each multiplet is computationally deconvoluted into its most likely single-cell constituents, based on its transcriptional profile and singlet cell types from the same tissue. Statistical enrichment of co-occurring cells in a large cohort of multiplets indicates physical interaction in the tissue. The results are summarized in the circos plot on the right, where cell classes are shown as colored blocks, connected by lines representing interactions, with the color of the line indicating whether the interaction is significant or not significant (NS) (purple or gray), as well as the fold enrichment of significant interactions. The width of the line connecting to a cell class is proportional to the relative frequency of that connection and the height of the boxes at the circumference is proportional to the frequency of cell types. Obs., observed; exp., expected.