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GENE EXPRESSION

Expanding views of the transcriptome

Nature Methods volume 18, page 332 (2021)Cite this article

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In situ long-read sequencing combined with expansion microscopy enables precise views of transcriptomes in intact biological systems.

Since its inception, numerous extensions to ExM have shown the benefits of the technique for imaging a range of biomolecules, such as proteins, RNA, DNA and lipids, in diverse and challenging sample types, including intact C. elegans and formaldehyde-fixed and paraffin-embedded clinical samples. And it has become clear that this combination of sample de-crowding, optical clearing and compatibility with standard microscopes enables new investigations of biological structures.

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Research paper

  • Alon, S. et al. Expansion sequencing: spatially precise in situ transcriptomics in intact biological systems. Science 371, eaax2656 (2021).

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  1. Nature Methods http://www.nature.com/nmeth

    Rita Strack

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  1. Rita Strack
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Correspondence to Rita Strack.

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Strack, R. Expanding views of the transcriptome. Nat Methods 18, 332 (2021). https://doi.org/10.1038/s41592-021-01119-1

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  • DOI: https://doi.org/10.1038/s41592-021-01119-1

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