Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

METLIN MS2 molecular standards database: a broad chemical and biological resource

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: The METLIN MS2 database.

Data availability

The data in the METLIN database are available at The data in other databases mentioned in this study were obtained from their websites (accessed in February 2020) or published papers: MoNA (, mzCloud (, GNPS (, HMDB ( and NIST 17 (


  1. Guijas, C. et al. Anal. Chem. 90, 3156–3164 (2018).

    Article  CAS  Google Scholar 

  2. Tautenhahn, R. et al. Nat. Biotechnol. 30, 826–828 (2012).

    Article  CAS  Google Scholar 

  3. Kafader, J. O. et al. Nat. Methods 17, 391–394 (2020).

    Article  CAS  Google Scholar 

  4. Benton, H. P., Wong, D. M., Trauger, S. A. & Siuzdak, G. Anal. Chem. 80, 6382–6389 (2008).

    Article  CAS  Google Scholar 

  5. Flasch, M. et al. ACS Chem. Biol. 15, 970–981 (2020).

    Article  CAS  Google Scholar 

  6. Xue, J. et al. Anal. Chem. 92, 6051–6059 (2020).

    Article  CAS  Google Scholar 

  7. Quinn, R. A. et al. Nature 579, 123–129 (2020).

    Article  CAS  Google Scholar 

  8. Clayton, T. A., Baker, D., Lindon, J. C., Everett, J. R. & Nicholson, J. K. Proc. Natl Acad. Sci. USA 106, 14728–14733 (2009).

    Article  CAS  Google Scholar 

  9. Wang, M. et al. Nat. Biotechnol. 38, 23–26 (2020).

    Article  CAS  Google Scholar 

  10. Wishart, D. S. et al. Nucleic Acids Res. 46, D608–D617 (2018). D1.

    Article  CAS  Google Scholar 

Download references


This research was partially funded by US National Institutes of Health grants R35 GM130385 (G.S.), P30 MH062261 (G.S.), P01 DA026146 (G.S.) and U01 CA235493 (G.S.) and by Ecosystems and Networks Integrated with Genes and Molecular Assemblies (ENIGMA), a Scientific Focus Area Program at Lawrence Berkeley National Laboratory for the US Department of Energy, Office of Science, Office of Biological and Environmental Research, under contract number DE-AC02-05CH11231 (G.S.).

Author information

Authors and Affiliations



J.X., C.G., H.P.B., B.W. and G.S. contributed to data collection, analysis, and manuscript writing. J.X. and C.G. share first authorship.

Corresponding author

Correspondence to Gary Siuzdak.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Editorial note: This article has been peer reviewed.

Source data

Source Data Fig. 1

Statistical Source Data

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xue, J., Guijas, C., Benton, H.P. et al. METLIN MS2 molecular standards database: a broad chemical and biological resource. Nat Methods 17, 953–954 (2020).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research