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SMAP: a modular super-resolution microscopy analysis platform for SMLM data

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Fig. 1: Overview of SMAP.

Code availability

The source code can be found at http://www.github.com/jries/SMAP. A standalone version for PC or Mac is available as Supplementary Software. The standalone version, as well as documentation, test data and updated versions, can be downloaded at http://www.rieslab.de.

References

  1. Betzig, E. et al. Science 313, 1642–1645 (2006).

    Article  Google Scholar 

  2. Rust, M. J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    Article  CAS  Google Scholar 

  3. Jungmann, R. et al. Nat. Methods 11, 313–318 (2014).

    Article  CAS  Google Scholar 

  4. Henriques, R. et al. Nat. Methods 7, 339–340 (2010).

    Article  CAS  Google Scholar 

  5. Wolter, S. et al. Nat. Methods 9, 1040–1041 (2012).

    Article  CAS  Google Scholar 

  6. Dedecker, P., Duwé, S., Neely, R. K. & Zhang, J. J. Biomed. Opt. 17, 126008 (2012).

    Article  Google Scholar 

  7. Ovesný, M., Křížek, P., Borkovec, J., Švindrych, Z. & Hagen, G. M. Bioinformatics 30, 2389–2390 (2014).

    Article  CAS  Google Scholar 

  8. Schnitzbauer, J., Strauss, M. T., Schlichthaerle, T., Schueder, F. & Jungmann, R. Nat. Protoc. 12, 1198–1228 (2017).

    Article  CAS  Google Scholar 

  9. Sage, D. et al. Nat. Methods 12, 717–724 (2015).

    Article  CAS  Google Scholar 

  10. Li, Y. et al. Nat. Methods 15, 367–369 (2018).

    Article  CAS  Google Scholar 

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Acknowledgements

We thank I. Schön, E. Klotzsch, all members of the Ries group and specifically J. Deschamps, M. Mund, U. Matti, P. Hoess Y.L. Wu, S. Liu, T. Deguchi and R. Diekmann for extensive testing of the software and contributions to the manuscript and software. This work was supported by the European Research Council (ERC CoG-724489) and the European Molecular Biology Laboratory.

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Correspondence to Jonas Ries.

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The author declares no competing interests.

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Editorial note: This article has been peer reviewed.

Supplementary information

Supplementary Table

List of plugins currently provided with SMAP

Supplementary Software

Compiled standalone versions of SMAP for PC and Mac

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Ries, J. SMAP: a modular super-resolution microscopy analysis platform for SMLM data. Nat Methods 17, 870–872 (2020). https://doi.org/10.1038/s41592-020-0938-1

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