Fig. 1: Development and validation of scNT-seq. | Nature Methods

Fig. 1: Development and validation of scNT-seq.

From: Massively parallel and time-resolved RNA sequencing in single cells with scNT-seq

Fig. 1

a, Overview of scNT-seq. TSO, template switch oligo. b, Species-mixing experiment benchmarks the performance of TFEA/NaIO4- and IAA-based chemical conversion reactions on pooled beads in scNT-seq, by sequencing a mix (1:1) of human K562 cells and mESCs. The scatterplot shows the number of transcripts (UMIs) mapped to the mouse (y axis) or human (x axis) genome for each cell (dot) that is colored by its identity (human, blue; mouse, red; mixed, green). c, Bar plot showing nucleotide substitution rates in 4sU-labeled K562 cells. Untreated, control cells that were not chemically treated were used. d, Box plots showing the fraction of 4sU-labeled transcripts (UMI) per cell in untreated (n = 193 cells) and TFEA/NaIO4-treated (n = 202 cells) K562 cells. See ‘Data visualization’ in the Methods for definitions of box plot elements.

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