Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

SEQUENCING

Mapping DNA single-strand breaks

Sriramachandran, A. M. et al. Mol. Cell https://doi.org/10.1016/j.molcel.2020.03.027 (2020).

Sequencing-based methods have been developed to detect DNA damage events and reveal their genome-wide distribution. Single-strand breaks (SSBs) are one of the most frequent types of DNA damage in the genome and have been profiled by methods involving poly(A) tailing or labeling during nick translation, which may reduce the resolution of the original position of break sites. To obtain a map with nucleotide resolution, Sriramachandran et al. developed genome-wide ligation of 3′-OH ends followed by sequencing (GLOE-Seq), which detects free 3′-OH termini resulted from SSBs, lesions or other repair intermediates. In GLOE-Seq, genomic DNA is heat-denatured and ligated to a biotinylated adapter with the assistance of a splinter oligonucleotide, followed by fragmentation and capture on streptavidin beads. GLOE-Seq has been applied in mapping DNA SSBs and lesions in yeast and human chromatin. The researchers also provide a software pipeline for annotating and visualizing strand breaks.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Lei Tang.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Tang, L. Mapping DNA single-strand breaks. Nat Methods 17, 564 (2020). https://doi.org/10.1038/s41592-020-0876-y

Download citation

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing