Mapping spatial diversity

Bayraktar, O. E. et al. Nat. Neurosci. 23, 500–509 (2020).

The layered organization of the cortex is well established for mammalian brains, but whether astrocytes exhibit spatial heterogeneity has been unclear. To assess spatial diversity, Bayraktar et al. have established a pipeline consisting of multiplexed single-molecule fluorescence in situ hybridization, high-resolution imaging, cell segmentation and quantification. To analyze astrocyte diversity in the mouse cortex, the researchers first identified candidate genes by dissecting the upper and lower cortex, purifying astrocytes and performing transcriptomic analysis. Using their pipeline, the authors then established that astrocytes formed three layers that were not congruent with neuronal layers. Furthermore, the researchers observed differences in layered gene expression in different cortical areas.

Author information



Corresponding author

Correspondence to Nina Vogt.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Vogt, N. Mapping spatial diversity. Nat Methods 17, 461 (2020).

Download citation


Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing