Extended Data Fig. 4: 3P Axial resolution measurement. | Nature Methods

Extended Data Fig. 4: 3P Axial resolution measurement.

From: Deep three-photon imaging of the brain in intact adult zebrafish

Extended Data Fig. 4

a, 3P image of a small fluorescein-labeled blood vessel (blue arrowhead) imaged about 200 μm deep into the optic tectum. Image stack was taken with 0.3 μm axial steps at 3 µs pixel time (512x512 pixels/frame) and 4 times averaging. b, Intensity profile along the axial direction fitted with a Gaussian curve to characterize the upper bound of axial resolution. The full width at half maximum of the Gaussian fit is 2.9 μm. N=1.

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