Extended Data Fig. 3: Signal to Background ratio. | Nature Methods

Extended Data Fig. 3: Signal to Background ratio.

From: Deep three-photon imaging of the brain in intact adult zebrafish

Extended Data Fig. 3

Signal-to-background ratio (SBR) in shallow and deep regions in three areas of the brain. ac, Fluorescence intensity profiles along the lines across GCaMP6s-labeled neuronal nuclei (N=1 each). df, Fluorescence intensity profiles along the lines across blood vessels labelled with fluorescein (N=2 each). To produce each plot, a five-pixel-wide line was drawn across the blood vessel or nucleus. The 5 pixel values were averaged to get an intensity value for the corresponding position along the line. The x axis corresponds to the lines shown on the images to the right. Scale bar 50 μm.

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