MICROBIOLOGY

A genetic toolbox for marine protists

A resource of detailed DNA delivery and expression protocols for marine protists will enable new studies to understand the fundamental and ancestral features of eukaryotic cells.

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Fig. 1: Genetic transformation across the tree of eukaryotes.

References

  1. 1.

    Faktorová, D. et al. Nat. Methods https://doi.org/10.1038/s41592-020-0796-x (2020).

  2. 2.

    Worden, A. Z. et al. Science 347, 1257594 (2015).

    Article  Google Scholar 

  3. 3.

    Bar-On, Y. M., Phillips, R. & Milo, R. Proc. Natl Acad. Sci. USA 115, 6506–6511 (2018).

    CAS  Article  Google Scholar 

  4. 4.

    de Vargas, C. et al. Science 348, 1261605 (2015).

    Article  Google Scholar 

  5. 5.

    Simpson, A. G. B. & Roger, A. J. Curr. Biol. 14, R693–R696 (2004).

    CAS  Article  Google Scholar 

  6. 6.

    Brown, M. W. et al. Genome Biol. Evol. 10, 427–433 (2018).

    CAS  Article  Google Scholar 

  7. 7.

    Burki, F., Roger, A. J., Brown, M. W. & Simpson, A. G. B. Trends Ecol. Evol. 35, 43–55 (2019).

    Article  Google Scholar 

  8. 8.

    Ruiz-Trillo, I. et al. Curr. Biol. 23, 113–118 (2007).

    CAS  Google Scholar 

  9. 9.

    Keeling, P. J. et al. PLoS Biol. 12, e1001889 (2014).

    Article  Google Scholar 

  10. 10.

    Hinnen, A., Hicks, J. B. & Fink, G. R. Proc. Natl Acad. Sci. USA 75, 1929–1933 (1978).

    CAS  Article  Google Scholar 

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Correspondence to Matthew W. Brown.

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Brown, M.W., Tice, A.K. A genetic toolbox for marine protists. Nat Methods 17, 469–470 (2020). https://doi.org/10.1038/s41592-020-0794-z

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