Understanding how chromatin is regulated is essential to fully grasp genome biology, and establishing the locus-specific protein composition is a major step toward this goal. Here we explain why the isolation and analysis of a specific chromatin segment are technically challenging, independently of the method. We then describe the published strategies and discuss their advantages and limitations. We conclude by discussing why significant technology developments are required to unambiguously describe the composition of small single loci.
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Work in the Déjardin lab is supported by grants from ARC équipe labelisée 2016, from the ERC CoG METACHROM, from INCA, from the Fondation Schlumberger pour l’éducation et la recherche and from Merck (MSD Gnostic). M.G. is supported by the University of Montpellier and by ARC. The Vermeulen lab is part of the Oncode Institute, which is partly funded by the Dutch Cancer Society.
The authors declare no competing interests.
Peer review information Rita Strack was the primary editor on this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.
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Gauchier, M., van Mierlo, G., Vermeulen, M. et al. Purification and enrichment of specific chromatin loci. Nat Methods 17, 380–389 (2020). https://doi.org/10.1038/s41592-020-0765-4
Frontiers in Cell and Developmental Biology (2020)
Nature Reviews Molecular Cell Biology (2020)