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Sensors and Probes

Calcium imaging in the NIR

Nature Methods volume 18, page 32 (2021)Cite this article

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The near-infrared calcium sensor iGECI shows promise for imaging neuronal activity in vivo.

Vladislav Verkhusha from the Albert Einstein College of Medicine in New York and the University of Helsinki and his collaborators have developed a NIR GECI called iGECI. He says that “we started from the classical Cameleon scaffold,” which consists of two fluorescent proteins linked by a calcium-binding module to form a fluorescence resonance energy transfer (FRET)-based biosensor. In the case of iGECI, the fluorescent proteins are miRFP670 as the FRET donor and miRFP720 as the FRET acceptor.

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  1. Nature Methods http://www.nature.com/nmeth

    Nina Vogt

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  1. Nina Vogt
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Correspondence to Nina Vogt.

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Vogt, N. Calcium imaging in the NIR. Nat Methods 18, 32 (2021). https://doi.org/10.1038/s41592-020-01044-9

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  • DOI: https://doi.org/10.1038/s41592-020-01044-9

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