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Single-objective light sheet microscopy

Nature Methods volume 18, page 28 (2021)Cite this article

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Single-objective light sheet fluorescence microscopes are driving innovation in volumetric imaging.

Geometry, however, has presented a challenge for light sheet fluorescence microscopy. Most conventional light sheet microscopes have two objectives arranged perpendicularly to one another, one for illuminating the sample and one for collecting emitted light. The size and orientation of these objectives restricts the types of sample that can be mounted between the objectives. This has led to creative strategies for mounting diverse samples and alternative instrument designs, including light sheet microscopes that use the same objective for illumination and detection.

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Change history

  • 18 January 2021

    In the version of this article initially published online, “Nat. Methods 16, 1054–1062, 2019” was linked to the wrong article. The error has been corrected in the PDF and HTML versions of the article.

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  1. Nature Methods http://www.nature.com/nmeth

    Rita Strack

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  1. Rita Strack
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Correspondence to Rita Strack.

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Strack, R. Single-objective light sheet microscopy. Nat Methods 18, 28 (2021). https://doi.org/10.1038/s41592-020-01027-w

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