Supplementary Fig. 1: Layout of the microfluidic chip. | Nature Methods

Supplementary Fig. 1: Layout of the microfluidic chip.

From: Time-resolved imaging-based CRISPRi screening

Supplementary Fig. 1

Image adapted from (Baltekin et al. 201713). The holes punched in the PDMS before bonding it to the PDMS are 2.0 (media in, front channel), 7.0 and 8.0 (waste port, front channel), 5.1 and 5.2 (waste port, back channel), 2.1 and 2.2 (cell loading or waste port, front channel).

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