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MICROSCOPY

Resolution upgrades for light-sheet microscopy

Nature Methods volume 16, pages 813–814 (2019)Cite this article

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An Author Correction to this article was published on 13 September 2019

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Single objective light-sheet fluorescence microscopes combine the convenience of conventional sample mounting with sensitive subcellular and super-resolution imaging of cells and tissues.

Using a single objective for light-sheet generation and fluorescence detection has the potential to make light-sheet fluorescence microscopy (LSFM) more user-friendly and widespread. Such systems can be integrated into existing epifluorescence microscope stands and are compatible with standard sample mounting, including multi-well plates. In contrast, conventional LSFM usually requires a dedicated instrument with specialized sample mounting.

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Fig. 1: Working principles of single objective LSFM.

Marina Corral Spence, Springer Nature (a–d); Andrew G. York, Calico Labs (e)

Change history

  • 13 September 2019

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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Authors and Affiliations

  1. Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Reto Fiolka

  2. Lydia Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Reto Fiolka

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  1. Reto Fiolka
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Correspondence to Reto Fiolka.

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Fiolka, R. Resolution upgrades for light-sheet microscopy. Nat Methods 16, 813–814 (2019). https://doi.org/10.1038/s41592-019-0542-4

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