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Resolution upgrades for light-sheet microscopy

An Author Correction to this article was published on 13 September 2019

This article has been updated

Single objective light-sheet fluorescence microscopes combine the convenience of conventional sample mounting with sensitive subcellular and super-resolution imaging of cells and tissues.

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Fig. 1: Working principles of single objective LSFM.

Marina Corral Spence, Springer Nature (ad); Andrew G. York, Calico Labs (e)

Change history

  • 13 September 2019

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.


  1. Dunsby, C. Opt. Express 16, 20306–20316 (2008).

    Article  CAS  Google Scholar 

  2. Bouchard, M. B. et al. Nat. Photon. 9, 113 (2015).

    Article  CAS  Google Scholar 

  3. Botcherby, E. J. et al. Opt. Letters 32, 2007–2009 (2007).

    Article  Google Scholar 

  4. Kim, J. et al. Nat. Methods (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Yang, B. et al. Nat. Methods 16, 501–504 (2019).

    Article  CAS  Google Scholar 

  6. Chen, B.-C. et al. Science 346, 1257998 (2014).

    Article  Google Scholar 

  7. Millett-Sikking, A. & York, A. G. Zenodo, (2019).

Download references

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Correspondence to Reto Fiolka.

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Fiolka, R. Resolution upgrades for light-sheet microscopy. Nat Methods 16, 813–814 (2019).

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