a–c, Surface renderings of a dendritic cell expressing Lifeact-GFP (a), an MV3 melanoma cell expressing tractin-GFP (b) and a HBEC expressing tractin-GFP (c). d–f, Maximum intensity projections (MIPs) of the cells shown in a–c, using an inverse look-up table. a–f are shown at the same scale. Additional views of these cells are shown in Supplementary Fig. 1. g, A MIP of a branched MV3 cells expressing PLCΔ-PH-GFP, a PIP2 translocation biosensor. h, A surface rendering of the same cell. Surface regions with relatively high PIP2 localization are shown in red, whereas regions of relatively low localization are shown in blue. i,j, A MIP (i) and a surface rendering of a blebbing MV3 cell expressing PLCΔ-PH-GFP (j). The PLCΔ-PH-GFP images are representative of 23 cells from three experiments. k, A MIP of an MV3 cell expressing GFP-KrasV12. l, A surface rendering of k. Surface regions of relatively high Kras localization are shown in red, whereas regions of relatively low localization are shown in blue. The GFP-KrasV12 images are representative of 31 cells from seven experiments. Scale bars, 10 μm.